Transmission-blocking compound candidates against Plasmodium vivax using P. berghei as an initial screening

Background Different strategies for improvement of malaria control and elimination are based on the blockage parasite transmission to mosquito vector. These include drugs that target plasmodial sexual stages in humans early developmental inside mosquitoes. Objectives Here we tested Malaria Box compounds order evaluate their activity against male female gametocytes Plasmodium berghei, infection P. vivax ookinete formation both species. Methods/findings The membrane feeding assay development ookinetes by a 24 h ex vivo culture yield per 1000 erythrocytes were used test transmission-blocking potential vivax. For berghei flow cytometry gametocyte time course fluorescence microscopy check development. two species this study showed similar results concerning compounds' ookinetes, which different from those falciparum. In addition, eight species, MMV665830, MMV665878 MMV665941 selected as hit due high inhibition observed. Conclusion Our is suitable an initial screening system